产品介绍
NCM Universal Antibody Diluent(通用型抗体稀释液)是一种即用型的,适合于稀释所 有免疫抗体测定(Immunofluorescence, IHC, ELISA, WB)实验中的抗体。该试剂可用于一抗 或二抗的稀释和配制,在4℃保存和使用不少于六个月;稀释液中加入了多种抗体结合反应 增强剂及稳定剂,增强免疫反应,减少非特异性结合,获得更佳的效果,可反复使用,节约 宝贵的抗体。试剂中不含有动物源的蛋白、磷酸盐、叠氮钠或硫柳汞类防腐剂,适合于所有 类型抗体稀释,包括 HRP,AP 标记的抗体。
产品特色
即用型稀释液,无需配制
适用所有抗体稀释(IF、IHC、ELISA、WB)
加入抗体结合反应增强剂,效果更佳
不含 BSA、脱脂奶粉等动物源蛋白
不含磷酸盐、叠氮钠或硫柳汞类防腐剂
操作步骤
根据抗体使用说明书,直接用本品对一抗或二抗进行适当倍数的稀释。保存在 NCM Universal Antibody Diluent 稀释液中的抗体可回收于 4℃保存,至少可反复多次使用 长达 6 个月以上,直至不能达到理想结果后丢弃。
保存条件
4℃保存,冰袋运输,至少 1年有效;
注意事项
1. 孵育抗体时尽量在 4 ºC 进行,可延长稀释后抗体的使用寿命; 2. 本品不含叠氮钠或硫柳汞类防腐剂,但含安全性更高的防腐剂,还需小心操作避免皮肤 直接接触。
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