产品介绍
NcmECL Ultra是新赛美生物科技有限公司研发的超敏ECL（Enhanced Chemiluminescent） 化学发光试剂，用于检测 HRP（辣根过氧化物酶）标记的目的条带，检测灵敏度最高可达飞 克（fg）级。NcmECL Ultra 优化了底物的成分，应用了新型的 Enhancer 和氧化剂，发光强度 更高，稳定性更好，不含有毒试剂，安全性更高。
产品特色
高灵敏度：最高可达飞克级别，10-15 g 安全性高：试剂不含对人体健康有毒的化学物质 信号持续时间长：发光时间长达 30 分钟以上 稳定性高：室温运输稳定性好 经济节约：较同类进口试剂花费更少，所需抗体少，抗体稀释度高
操作步骤
1. 准备工作稀释液，将 NcmECL Ultra Luminol/Enhancer Reagent (A) 和 NcmECL Ultra Stabilized Peroxide Reagent (B) 按体积比例 1:1 混合 注：为了获得最佳效果，使用前准备稀释液并立即使用。吸取 NcmECL Ultra 不同 组份时要换移液管，以免污染试剂。 2. 从 TBST 或 PBST 缓冲液的托盘中取出膜，小心移去膜上的多余缓冲液，但不要让膜干燥。 3. 将膜上有蛋白的一面朝上平整地铺在一张纸板上，加上配好的工作稀释液。用量以覆 盖膜为基准，1ml 工作液可以覆盖大约 10cm2的膜。 4. 将膜与工作稀释液孵育 1-5min，以确保整个表面覆盖。 5. 通过放射自显影胶片或成像设备获取信号。对于一个未知的信号，可调节不同曝光的 时间来获得最佳结果。
保存条件
室温运输，请于 4℃保存，有效期为 2 年。
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