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蛋白/免疫学系列产品

超敏ECL化学发光试剂盒

NcmECL Ultra

货号:
P10300
规格:
A液250ml;B液250ml
目录价:
¥1500.00
购买数量
+ -

产品介绍 

NcmECL Ultra是新赛美生物科技有限公司研发的超敏ECLEnhanced Chemiluminescent) 化学发光试剂,用于检测 HRP(辣根过氧化物酶)标记的目的条带,检测灵敏度最高可达飞 克(fg)级。NcmECL Ultra 优化了底物的成分,应用了新型的 Enhancer 和氧化剂,发光强度 更高,稳定性更好,不含有毒试剂,安全性更高。 

产品特色

 高灵敏度:最高可达飞克级别,10-15  安全性高:试剂不含对人体健康有毒的化学物质  信号持续时间长:发光时间长达 30 分钟以上  稳定性高:室温运输稳定性好  经济节约:较同类进口试剂花费更少,所需抗体少,抗体稀释度高 

操作步骤

1. 准备工作稀释液,将 NcmECL Ultra Luminol/Enhancer Reagent (A) 和 NcmECL Ultra  Stabilized Peroxide Reagent (B) 按体积比例 1:1 混合 注:为了获得最佳效果,使用前准备稀释液并立即使用。吸取 NcmECL Ultra 不同 组份时要换移液管,以免污染试剂。 2. 从 TBST 或 PBST 缓冲液的托盘中取出膜,小心移去膜上的多余缓冲液,但不要让膜干燥。 3. 将膜上有蛋白的一面朝上平整地铺在一张纸板上,加上配好的工作稀释液。用量以覆  盖膜为基准,1ml 工作液可以覆盖大约 10cm2的膜。 4. 将膜与工作稀释液孵育 1-5min,以确保整个表面覆盖。 5. 通过放射自显影胶片或成像设备获取信号。对于一个未知的信号,可调节不同曝光的  时间来获得最佳结果。

保存条件

室温运输,请于 4℃保存,有效期为 2 年。

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